這款產品是一種專為人類 iPSC 衍生心肌細胞設計的高效且溫和的細胞解離試劑。適用於透過單層培養法或胚體(embryoid body, EB)形成法分化而成的心肌細胞。此外,對於一般較難分散的心肌細胞聚集體,本產品亦能有效進行解離。
1. 造血幹細胞擴增培養評估
Human peripheral blood mononuclear cells (PBMCs) were cultured for five days in this medium supplemented with cytokines. After cell counting, expression of CD34 and CD45 was analyzed by flow cytometry. The results demonstrate that this medium efficiently supports the expansion of hematopoietic stem cells (CD45dim CD34+).
Cell culture was performed with reference to a protocol of CiRA Foundation.
https://www.cira-foundation.or.jp/j/assets/file/publications/EpisomalVector_iPS_protocol.pdf

2. iPS Cell Derivation
Human peripheral blood mononuclear cells (PBMCs) were cultured in this medium supplemented with six cytokines, followed by generation of iPS cells based on the RNA reprogramming method1.
