AscleStem® Cardiomyocyte Differentiation Medium（#13166-05）、Supplement A for C.D. Medium（#15345-24）及 Supplement B for C.D. Medium（#15346-14）為無血清培養基及無血清添加劑，用於誘導人類多能幹細胞分化為心肌細胞。

本培養流程以小分子化合物取代細胞激素（cytokines），有望降低培養成本，並可同時適用於單層培養法（貼附培養）及類胚體形成法（懸浮培養）。

1. Cell morphology and immunostaining results after differentiation 

Human iPSC cell lines were differentiated into cardiomyocytes using two culture methods: the monolayer culture method and the embryoid body formation method. Expression of the cardiomyocyte marker cTnT was confirmed by immunostaining using purified cells.

2. Response of cardiomyocytes differentiated by two culture methods to isoproterenol
The responsiveness of cardiomyocytes differentiated from human iPS cells by two culture methods (monolayer culture and embryoid body formation) to isoproterenol was evaluated using the SI8000 Cell Motion Imaging System (Sony). Similar results were obtained with both methods.