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Shows amplification traces of the ACTG1 gene from a dilution series of total RNA extracted from mouse liver. Total RNA concentration varied from 25pg to 250ng per 20μl reaction. Cycling conditions were 45°C 10 minutes for cDNA synthesis, followed by 95°C 2 minutes hot start, then 45 cycles of 95°C 10sec, 60°C 10sec on Roche LC480. qPCRBIO SyGreen 1-Step Go had equal performance at high RNA concentrations and superior performance at lower RNA concentrations, displaying linear spacing between amplification curves, earlier amplification by 3–4 cycles, and lower prevalence of primer dimer. |
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