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Four mouse housekeeping genes were amplified simultaneously in a single multiplex reaction. 1μg of mouse liver total RNA was used as template. Amplification was detected using TaqMan probes in the following gene/probe combinations: B2MG/HEX, ACTB/Cy5, GAPDH/FAM, and ACTG/TexasRed. Cycling conditions were 45°C 10min, 95°C 3min, then 45 cycles of 95°C 10s, 60°C 30s. This demonstrates that the qPCRBIO Probe 1-Step Go mix can be used to quantify and compare expression levels of multiple genes in a single reaction. |
