Products Description
源自嗜熱細菌的 Ampligase® Thermostable DNA Ligase 是具NAD+依賴性的熱穩定性連接酶 (thermostable ligase)，相較傳統 DNA 連接酶於高溫下更穩定且具活性。 其半衰期在 65°C 下為 48 小時，在 95°C 下 49 小時。已證實 Ampligase DNA Ligase 至少在 500 個熱循環 (94 °C/ 80 °C) 或 16 小時的循環中仍具有活性。
Ampligase DNA Ligase 對連接 blunt-ended DNA 以及 RNA 或 RNA:DNA hybrids 沒有檢測活性。
| Ampligase® DNA Ligase Version |
Sizes |
Provided Buffer |
| 低濃度 (5 U/μL) |
10,000 U |
without buffer |
| 2,500 U |
Ampligase® 10X DNA Ligase Reaction Buffer |
| 750 U |
Ampligase® 10X DNA Ligase Reaction Buffer |
| 高濃度 (100 U/µL) |
10,000 U |
without buffer |
| 2,500 U |
Ampligase® 10X DNA Ligase Reaction Buffer |
*可單獨購買Ampligase® 10X DNA Ligase Reaction Buffer
*另有Ampligase® DNA Ligase Kit : 1,000 U (5 U/μL)，含 buffer 及 Control DNA
適用於高溫、高嚴格的連接反應的應用：
− Ligation Amplification
− Repeat Expansion Detection
− High-Fidelity Gene Synthesis from overlapping oligodeoxynucleotides
− Multiple Site Mutagenesis
− Targeted Inverted Repeat Amplification
Reaction Buffer: 10X Ampligase Reaction Buffer contains 200 mM Tris-HCl (pH 8.3), 250 mM KCl, 100 mM MgCl2, 5 mM NAD, and 0.1% Triton® X-100.
Unit Definition: One unit of Ampligase Thermostable DNA Ligase catalyzes the ligation of 50% of the cos sites present in 1 μg of bacteriophage lambda DNA in 1 minute at 45°C.
*Note: One Ampligase unit is equivalent to at least 15 “cohesive end units” or the “nick closing units” of thermostable ligase described elsewhere.
Resources
Data Sheet
