來自德國的ChromoTek,以一類新的駱駝科動物特有的Heavy Chain Antibodies (hcAb)抗體為基礎,致力開發蛋白質體學和細胞影像領域相關產品。新開發的 HaloTag-Trap為高品質的HaloTag結合蛋白並直接連接到Agarose beads,適合用於生化分析, 尋找融合蛋白的作用蛋白, HaloTag為Rhodococcus rhodochrous 菌中 modified Haloalkane Dehalogenase 酵素。HaloTag-Trap可抓下結合/未結合HaloTag ligands 之HaloTag fusion proteins
產品特點:
1) 跑膠時無重鏈和輕鏈干擾
2) 親和力超高 (KD of 2nM)
3) 省時!5 – 30 min快速取得所要蛋白
4) 應用廣泛: Immunoprecipitation (IP) / Co-IP、Mass spectrometry、Enzyme activity measurements、ChIP / RIP analysis
Halo-Tag-fusion protein immunoprecipitated with HaloTag-Trap: Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie blue staining, Western blotting and fluorescence scan. Because the HaloTag-Trap binds to HaloTag at an epitope outside its catalytic center, both unbound HaloTag fusion proteins (left) and HaloTag fusions covalently bound to ligands like HaloTag TMR (right) are captured virtually without any difference. This allows to pulldown HaloTag fusion proteins from cells that have been previously treated with HaloTag ligands for imaging and makes the HaloTag system as versatile as GFP expression, imaging and purification. |
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