Signal Enhancer HIKARI for Western Blotting and ELISA

Cat.No. Size Price
02267-41 50+50 ml 來電價
02270-81 250+250 ml 來電價
02272-74 250 ml Solution A 來電價
02297-64 250 ml Solution B 來電價

針對Western blotting & ELISA 增強抗原抗體反應,信號提升數倍至數十倍 輕鬆去除背景,提高抗體的特異性 適用於各種substrate,增強化學發光與比色檢測 適用於各類膜材,提升NC膜與PVDF膜的信號 Ready to use

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使用 Signal Enhancer HIKARI 代替傳統稀釋劑(例如 PBS 或 TBS)稀釋抗體,使檢測目標蛋白質能力顯著提高並且消除不需要的背景雜訊。幫助解決 Western blotting 和 ELISA 等過程中經常遇到的低靈敏度和高背景問題。

Fig1. 用 HIKARI 處理後,Western blotting 中 Rac1 的檢測增強

 

Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500)
Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka,
Department of Psychiatry, Johns Hopkins University School of Medicine

Fig2. 使用 HIKARI 訊號增強劑可增強 磷酸化蛋白的檢測效果!

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico


Data Courtesy of Dr. S. Matsuzawa,
Signal Transduction, NCI Cancer Center, Burnham Institute for Medical Research

References

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