Ampligase® Thermostable DNA Ligase

Cat.No. Size Price
A1905B 5 mL 來電價

Cat. No. Sizes Component A3210K 10,000 U   Ampligase® DNA Ligase (5 U/μL)   A3202K 2,500 U   Ampligase® DNA Ligase (5 U/μL)  Ampligase® 10X DNA Ligase Reaction Buffer A32750 750 U   Ampligase® DNA Ligase (5 U/μL)  Ampligase® 10X DNA Ligase Reaction Buffer A0110K 10,000 U   Ampligase® DNA Ligase (100 U/μL)   A0102K 2,500 U   Ampligase® DNA Ligase (100 U/μL)  Ampligase® 10X DNA Ligase Reaction Buffer A8101 (Kit) 1,000 U   Ampligase® DNA Ligase (5 U/μL)  Ampligase® 10X DNA Ligase Reaction Buffer  Ampligase Ligation Control DNA (0.25 μg/μL) A1905B (Buffer) 5 mL   Ampligase® 10X DNA Ligase Reaction Buffer  

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源自嗜熱細菌的 Ampligase® Thermostable DNA Ligase 是具NAD+依賴性的熱穩定性連接酶 (thermostable ligase),相較傳統 DNA 連接酶於高溫下更穩定且具活性。 其半衰期在 65°C 下為 48 小時,在 95°C 下 49 小時。已證實 Ampligase DNA Ligase 至少在 500 個熱循環 (94 °C/ 80 °C) 或 16 小時的循環中仍具有活性。
Ampligase DNA Ligase 對連接 blunt-ended DNA 以及 RNA 或 RNA:DNA hybrids 沒有檢測活性。

 
Ampligase® DNA Ligase Version Sizes Provided Buffer
低濃度 (5 U/μL) 10,000 U   without buffer
2,500 U   Ampligase® 10X DNA Ligase Reaction Buffer
750 U   Ampligase® 10X DNA Ligase Reaction Buffer
高濃度 (100 U/µL) 10,000 U   without buffer
2,500 U   Ampligase® 10X DNA Ligase Reaction Buffer
*可單獨購買Ampligase® 10X DNA Ligase Reaction Buffer
*另有Ampligase® DNA Ligase Kit : 1,000 U (5 U/μL),含 buffer 及 Control DNA


適用於高溫、高嚴格的連接反應的應用:
− Ligation Amplification
− Repeat Expansion Detection
− High-Fidelity Gene Synthesis from overlapping oligodeoxynucleotides
− Multiple Site Mutagenesis
− Targeted Inverted Repeat Amplification

Reaction Buffer: 10X Ampligase Reaction Buffer contains 200 mM Tris-HCl (pH 8.3), 250 mM KCl, 100 mM MgCl2, 5 mM NAD, and 0.1% Triton® X-100.

Unit Definition: One unit of Ampligase Thermostable DNA Ligase catalyzes the ligation of 50% of the cos sites present in 1 μg of bacteriophage lambda DNA in 1 minute at 45°C.
*Note: One Ampligase unit is equivalent to at least 15 “cohesive end units” or the “nick closing units” of thermostable ligase described elsewhere.
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Data Sheet
Data Sheet
Data Sheet 2022-09-14 15:15:40

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