Products Description
Overview
- Ready-to-pour SDS polyacrylamide solutions - just add APS and TEMED
- Faster gel casting with no stacking gel required
- Broad range of separation - 3.5 kDa to 212 kDa on the same gel
- High resolution of protein bands
Detailed Description
NEXT GEL® products for denaturing gel electrophoresis are proprietary, ready-to-pour solutions comprised of acrylamide, bis-acrylamide, gel buffer and SDS. The unique chemistry of NEXT GEL® eliminates the need for a stacking gel, thus reducing gel preparation time and extending the separation matrix available for electrophoresis, enabling resolution of small peptides and high molecular weight proteins in the same gel.
NEXT GEL® solutions polymerize upon addition of ammonium persulfate and TEMED and are fully compatible with all standard electrophoresis equipment, SDS-PAGE staining procedures and downstream applications including Western blotting, protein sequencing and MALDI analysis. Each NEXT GEL® acrylamide solution is supplied with NEXT GEL® Running Buffer, 20X, which is essential for optimal gel performance.
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Information
Resolution of a wide range of proteins on a 10% NEXT GEL®. Low (K880, lane 1), Mid/Low (J450, lane 2), and Wide (K494, lane 3) Range Protein Molecular Weight Markers were loaded on a 10% NEXT GEL® individually and as a mixture of all three markers (lane 4). The gel was post-stained with Coomassie® Blue and destained using standard procedures. The NEXT GEL® shows resolution of proteins from 3.5 kDa to 212.0 kDa without the need for an acrylamide gradient.