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LavaLAMP™ DNA Master Mix

Cat.No. Size Price Quantity Subtotal Buy
30066-1 200 rxns 來電價
30067-1 with dye 200 rxns 來電價

Cat. No.: 30066, 30067

Products Description

特色:

  • 恆溫增幅反應,只需簡單的加熱設備即可
  • 簡化步驟, 減少需要優化的條件
  • 耐高溫(90°C, 5min),可預熱檢體以增加反應的 sensitivity
  • 可增加 primer 專一性,降低背景值
  • 冷凍乾燥後可室溫保存攜帶,適合田野分子流行病學調查
  • 其中30067-1 Mix內含螢光可直接 Real-Time 偵測

Figure 1. Simplified overview of the loop-mediated isothermal amplification (LAMP) process. For simplicity, 4/6 potential LAMP primers are illustrated. The inclusion of two additional primers, F-Loop; Forward Loop Primer and B-Loop; Backward Loop Primer, often significantly enhances amplification. For more information, please visit the Eiken website.
 
Figure 2. Illustration of the differences in LAMP reaction setup and potential optimization parameters when using a master mix vs. a component kit format. Panel A illustrates the components added to a LAMP reaction (left) when using the LavaLAMP DNA Master Mix and the various parameters than can be optimized (right). Panel B also illlustrates reaction setup (left) and potential optimization parameters (right) when using a standard LAMP component kit.
 
Figure 3. Loop-mediated isothermal amplification (LAMP) with real-time fluorescent detection of amplified products. LAMP reactions were set up using the indicated kits according to manufacturer’s recommendations. Target DNA (C. difficile) at varying input amounts, tcdA target LAMP primers, and Green Fluorescent Dye (LavaLAMP Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; other kits at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. NTC = No Target Control.
 
Figure 4. Testing the thermostability of the LavaLAMP™ DNA Master Mix. Two sets of triplicate LAMP reactions were set up using the indicated amounts of target DNA (C. difficile), tcdA target LAMP primers, and Green Fluorescent Dye. One set of reactions was preheated to 90°C for 5 minutes, and then all reactions were incubated for 60 minutes on a CFX96 Thermal Cycler (Bio-Rad) at 68°C and fluorescence was measured during the course of the reactions to determine the TTR. NTC = No Target Control.

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