Overview
Detailed Description
EZ-Vision® In-Gel Solution, 10,000X is a non-mutagenic, non-toxic fluorescent DNA Dye that is used as an in-gel or post-run stain to visualize DNA in agarose gels. EZ-Vision® In-Gel Solution is an excellent replacement for the often used, but hazardous ethidium bromide. As an in-gel preparation for electrophoresis, EZ-Vision® In-Gel Solution is simply added to molten agarose prior to gel casting, allowing instant visualization of DNA bands upon UV exposure. Alternatively, EZ-Vision® In-Gel Solution can be used to stain the gel after electrophoresis.
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Code |
Product Specifications |
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N391-0.5ML |
0.5 ml of solution in a 10,000X concentrate |
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N391-5MLDRP |
5 ml dropper bottle; non-concentrate* |
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N391-15MLDRP |
15 ml dropper bottle; non-concentrate* |
*Dropper bottle sizes are not in a 10000X concentrate.
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EZ-Vision® In-Gel Sensitivity. DNA (2.5 mg - 80 ng) was mixed with 6X Agarose Gel Loading Buffer and applied to a 1% TAE agarose gel. Samples were resolved for 1 hour at 6.4 V/cm in 1X TAE buffer. Fluorescent visualization of DNA was achieved with a 500 msec UV exposure using a SYBR® Green filter with the Syngene GBox-HR Gel Doc System.
EZ-Vision® In-Gel Detection of Different Sized DNA. DNA markers were mixed with 6X agarose gel loading buffer and applied to either a 2.5% (A) or a 0.6% (B) TAE agarose gel. AMRESCO's 100 bp Ladder (K180), DNA MicroMarker™ (E840) and PCR DNA Marker™ (E854) were loaded in lanes 1-3, respectively (A). AMRESCO's 1 kb Ladder (K181), Low Range I™ Marker (E261), Low Range II™ Marker (E683), and Low Range III™ Marker (E687) were loaded in lanes 1-4, respectively (B). Samples were resolved for one hour at 6.4 V/cm in 1X TAE buffer. Fluorescent visualization of DNA was achieved with a 600 msec UV exposure using a SYBR® Green filter with the Syngene GBox-HR Gel Doc System.
SDS