Target description:
The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.
Antigen:
Rabbit polyclonal antibody raised against a 6x histidine peptide conjugated to OVA.
Application tested:
Western Blotting: 1:1,000 - 1:10,000
ELISA: 2,000 ~ 20,000 dilution
IP: 500 ~ 1,000 dilution
Form: Liquid (containing 50% glycerol)
Storage:
Aliquot and store at -20º C for long term storage. The product is stable for one year from the date of shipment.
