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LavaLAMP™ RNA Component Kit

Cat.No. Size Price Quantity Subtotal Buy
30096-1 200 Reactions 來電價
30097-1 with dye 200 Reactions 來電價

Products Description

LavaLAMP™RNA組件試劑盒

使用這種可完全優化的RNA LAMP試劑盒進行靈敏,快速的RNA檢測

特色:
  • 等溫擴增:可使用簡單和低成本的加熱儀器進行擴增反應
  • 可冷凍乾造:所有試劑盒組分都是凍乾相容的,去除已知抑制凍乾的成分
  • 可優化:非Master Mix試劑盒型式可以由使用者自行優化反應條件,
  • LavaLAMP™RNA Master Mix的可相比擬的性能

Like the related, LavaLAMP™ RNA Master Mix, this component kit is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. With these unique features, it is possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) using a single DNA polymerase. This enzyme first copies the RNA into cDNA, and then loop mediated isothermal amplification takes over to amplify the initial RNA target sequence present in the new cDNA making amplified and detectable target-specific, dsDNA.

The LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are set up using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the recommended initial Master Mix reaction outline in its User Manual. The individual reagent format of this kit, as opposed to the master mix format, enables complete control of each LAMP assay allowing you to carefully identify the best reaction conditions for your specific target and primer set.

Equivalent Performance Between the LavaLAMP™ RNA Master Mix and RNA Component Kit

 
Figure 1. Comparing the performance of the LavaLAMP™ RNA Component Kit and the LavaLAMP™ RNA Master Mix. LAMP reactions (6 replicates per test) were set up using the indicated kits and targets (A, MS2 and B, Zika) with the same concentrations of Target-Specific Primer Mixes (1X) and the same target input amounts. The LavaLAMP RNA Component Kit reactions were formulated to 1X LavaLAMP RNA Buffer, 5 mM MgSO4 and 0.8 mM dNTPs (each) to match the final formulations of the LavaLAMP RNA Master Mix reactions. Green Fluorescent Dye was included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at 68°C for both the MS2 and Zika targets and fluorescence was measured during the course of the 60-minute reactions to determine TTR. NTC denotes No Target Control.
 

Faster, More Sensitive Detection with LavaLAMP™ RNA Component Kit


Figure 2. Performance comparison of the LavaLAMP™ RNA Component Kit vs. competitor kits. (A) RNA LAMP reactions were set up using the indicated kits according to manufacturer’s recommendations. Target MS2 RNA at varying input amounts, MS2 target RNA LAMP primers, and Green Fluorescent Dye (LavaLAMP Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; other polymerases at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. (B) Delta TTRs were calculated by subtracting the low target level TTR from the NTC TTR for each polymerase tested. Higher positive values indicate better signal to noise, and hence better performance. NTC denotes No Target Control.

Information

Both LavaLAMP™ RNA Component Kits contain: 10X LavaLAMP™ RNA Buffer, LavaLAMP™ RNA Enzyme, Magnesium Sulfate, 100 mM, RNA Positive Control LAMP Primer Mix and RNA Positive Control. The LavaLAMP™ RNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

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