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| Figure 1. Performance comparison of the LavaLAMP™ DNA Component Kit and the LavaLAMP™ DNA Master Mix. LAMP reactions (8 replicates per test) were set up using the indicated kits and targets with the same concentrations of Target-Specific Primer Mixes (1X) and the same target input amounts. The LavaLAMP DNA Component Kit reactions were formulated to 1X LavaLAMP DNA Buffer, 10 mM MgSO4 and 0.8mM dNTPs (each) to match the final formulations of the LavaLAMP DNA Master Mix reactions. Green Fluorescent Dye was included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures - M13mp18, 74°C and tcdA, 69°C and fluorescence was measured during the course of the 60-minute reactions to determine TTR. NTC = No Target Control. |
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| Figure 2. Performance comparison of the LavaLAMP™ DNA Component Kit vs. Competitor Kits. LAMP reactions were set up using the indicated kits according to manufacturer’s recommendations. Target DNA (C. difficile) at varying input amounts, tcdA target LAMP primers, and Green Fluorescent Dye (LavaLAMP Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 69°C; other kits at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. NTC = No Target Control. |
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| Figure 3. Analysis of operator to operator consistency. On the same day, six replicate LAMP reactions were set up per condition per by three different operators using the indicated amounts of target DNA M13mp18, M13mp18 target LAMP primers, and Green Fluorescent Dye. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at 74°C and amplification was monitored in real-time. Results were analyzed to determine the TTR and averaged. NTC = No Target Control. |
