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Products Description

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
 

Figure 1. Comparison of qPCRBIO SyGreen 1-Step Go (purple) against competitor Bioline (blue)
		Shows amplification traces of the ACTG1 gene from a dilution series of total RNA extracted from mouse liver. Total RNA concentration varied from 25pg to 250ng per 20μl reaction. Cycling conditions were 45°C 10 minutes for cDNA synthesis, followed by 95°C 2 minutes hot start, then 45 cycles of 95°C 10sec, 60°C 10sec on Roche LC480. qPCRBIO SyGreen 1-Step Go had equal performance at high RNA concentrations and superior performance at lower RNA concentrations, displaying linear spacing between amplification curves, earlier amplification by 3–4 cycles, and lower prevalence of primer dimer.

Figure 2. Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product
		The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. qPCRBIO SyGreen 1-Step Detect (blue) shows efficient amplification of total RNA in the range 10pg to 10ng per reaction (figure 2a). Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 30sec, concluding with a melt analysis (figure 2b) on a Roche LC480.