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Experiments 1, 2 and 3 show TaqMan probe amplification traces of human gene ACVR2B in singleplex and in quadplex (ACVR2B, LIMK1, ACVR1B and CDK7) from a cDNA dilution series. a) traces indicate singleplex reactions, b) traces indicate quadplex reactions. qPCRBIO Probe Mix was tested against the latest competitor mixes from Invitrogen (experiment 2) and Biorad (experiment 3). qPCRBIO Probe Mix shows the least PCR inhibition when in multiplex compared to Invitrogen and Biorad mixes. This is evident in more delayed amplification traces in quadplex (b) compared to singleplex (a). Experiments 4, 5 and 6 show TaqMan probe amplification traces of human gene LIMK1 in singleplex and quadplex (ACVR2B, LIMK1, ACVR1B and CDK7). As with experiments 1, 2 and 3 LIMK1 amplification is less inhibited in multiplex in the PCR Biosystems probe mix than the competitor mixes tested. Cycling conditions were 95°C 2min, 40 cycles of 95°C 10sec, 60°C 15sec on Biorad CFX instrument. Experiments 7, 8 and 9 show TaqMan probe amplification traces from plasmid dilution series of 1x106 copies to 10 copies of DNA. For each gene qPCRBIO Probe Mix amplified with 100% efficiency and detected 10 copies of DNA. |
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