Products Description
特色:
抗體的 HoT Start Taq + 優化的 buffer
加熱到達 95°C 才有活性,65°C 以下沒有活性,以避免 primer dimer 的產生和非專一性的擴增
適用於特別困難的 templates (long, GC (up to 80% GC) or AT rich, 微量 or contains PCR inhibitors)
適合用來進行 Low-copy-number templates 的 PCR 實驗。
比 Taq 高3倍的正確率
25°C 可保存4週
PCRBIO Ultra Polymerase 可在多種Templates提供卓越的PCR性能和更高的成功率,包含複雜的基因組DNA和GC含量高的目標(GC高達80%)。PCRBIO Ultra Polymerase對PCR抑製劑具有高耐受性,使其成為菌落挑選和快速萃取檢體其PCR的理想選擇。
PCRBIO Ultra Polymerase利用先進聚合酶技術和緩衝液的發展技術,提高PCR反應的速度,產量和特異性。利用抗體在PCR反應前的常温階段抑制polymerase活性,以提供Hot-Start功能,可防止Primer Dimer和非特異性產物的形成,從而能夠從低拷貝數靶序列進行特異性和靈敏的擴增。
為方便起見,PCRBIO Ultra Polymerase也有2x Master Mix,含有除了引物和模板外的所有組成成分。另外PCRBIO Ultra Mix Red含有紅色染料,適合在瓊脂糖凝膠電泳過程中直接Loading和追踪。
應用:
Difficult PCR - GC/AT rich DNA、Low copy template detection、Crude sample PCR、Colony PCR、Long range PCR、Multiplex PCR、TA cloning、Next generation re-sequencing
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Figure 1. Long PCR products visualised on agarose gel Amplification of 0.8kb, 1.0kb, 1.8kb, and 2.7kb fragments of the GAPDH gene, a 4.6kb fragment of the RBL15 gene, and a 5.9kb fragment of the MYH6 gene. The starting template amount is 20ng (50ng for the 5.9kb fragment) of mouse genomic DNA and is diluted 2 to 5 fold. PCRBIO Ultra Polymerase amplifies the range of fragment lengths indicated with high yield and specificity.
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Figure 2. GC rich products visualised on agarose gel Amplification of 0.5kb (P1) and 0.6kb (P2) fragments of the ATXN2 gene with GC contents of 69% and 71%, respectively, using 20ng of mouse genomic DNA as template and a range of annealing temperatures from 67°C to 60°C (B-E). PCRBIO Ultra Polymerase efficiently amplifies GC rich templates >65% GC and is recommended for templates up to 80% GC. |
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