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qScript cDNA Synthesis Kit

Cat.No. Size Price Quantity Subtotal Buy
95047-025 25 x 20 μL rxns (1 x 25 µL) 來電價
95047-100 100 x 20 μL rxns (1 x 100 µL) 來電價
95047-500 500 x 20 μL rxns (1 x 500 µL) 來電價

Features & Benefits

  • Economical 2-component kit ideally suited for high throughput expression studies
  • Optimized, double pre-primed master mix component ensures equal representation of 5' and 3' RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
  • Broad linear dynamic detection range with limiting (10pg) or plentiful (1ug) samples of total RNA

Products Description

Description

The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. This economical, highly stable stabilize, 2-component reagent system has been rigorously optimized to ensure sensitive and linear RNA detection with a wide-range of input RNA and relative abundance. Reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and assay performance consistency. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5' and 3'-sequences for accurate gene expression quantification. The resulting cDNA product is directly compatible with all qPCR chemistries or conventional end-point RT-PCR of amplified fragments ≤1 kb in length.

For gene-specific priming (GSP) or two-step RT-PCR of RNA exceeding 1kb in total length, see our qScript® Flex cDNA Kit.


Performance Data





 

Information

Content

  • 5X concentrated master mix containing: titered primer blend (oligo dT(20) and random hexamer), qPCR-optimized dNTP blend and flexible magnesium titration
  • 50X concentrated qScript reverse transcriptase
  • Nuclease-free water


Storage and Handling

Store components in a constant temperature freezer at -25°C to -15°C upon receipt. After thawing, mix thoroughly before using. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form. 
Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. Concentrated reagents are viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.

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