Products Description
Description
qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.
Information
Contents
Single-tube, 5X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- Recombinant ribonuclease inhibitor protein (RIP)
- qScript XLT reverse transcriptase
- Titrated concentrations of random hexamer and oligo(dT) primer
- Proprietary enzyme stabilizers and performance-enhancing additives
Storage and Handling:
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube.
5X concentrated reagent is viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.
User Manual