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波仕特生產與代理產品
常見問題集
Q3:
該如何選擇Amresco的Protease
inhibitor呢?其中成分又有何差異呢?
Ans:
Protease
inhibitor有General
use, Bacterial use, Mammalian use, Plant use,
可以依照實驗來做選擇.
挑選表格如下:
|
Product |
Code |
|
Description |
Size |
|
Protease Inhibitor Cocktail, General Use |
M221 |
General Use |
Each vial can be reconstituted in 1 ml deionized water to form a
100X solution. |
1.0
ml |
|
Protease Inhibitor Cocktail, General Use with EDTA
|
M222 |
General Use |
Supplied as lyophilized powder.
Each vial can be reconstituted in 1 ml deionized water to form a
100X solution. |
1.0
ml |
|
Protease Inhibitor Cocktail, Mammalian |
M250 |
Mammalian |
Supplied as lyophilized powder.
Each vial can be reconstituted in 1 ml deionized water to form a
100X solution. |
1.0
ml |
|
Protease Inhibitor Cocktail, Bacterial
|
M306 |
Bacterial |
Supplied as lyophilized powder.
Each vial contains sufficient material to provide 5 ml of a 20X
solution upon reconstitution. |
5.0
ml |
|
Protease Inhibitor Cocktail, Plant
|
M307 |
Plant |
Supplied as lyophilized powder.
Each vial can be reconstituted in 1 ml dimethyl sulfoxide to
form a 100X solution. |
1.0
ml |
各成分如下:
M221-1ML, General Use Protease Inhibitor Cocktail
M222-1ML, General Use Protease Inhibitor Cocktail with EDTA
Preparation of 100X Solution:
1. Add 1 ml deionized, distilled water and mix gently until powder is
completely resuspended.
2. Aliquot into multiple tubes and store at -20ºC.
|
Name |
MW |
100X Concentration |
Recommended
Working Concentration |
|
AEBSF |
239.5 |
50.00 mM |
0.50 mM |
|
Aprotinin |
6512.0 |
30.00 uM |
0.30 uM |
|
Bestatin |
308.4 |
1.00 mM |
10.00 uM |
|
E-64 |
357.4 |
1.00 mM |
10.00 uM |
|
Leupeptin |
493.6 |
1.00 mM |
10.00 uM |
|
EDTA* |
372.2 |
5.00 mM |
50.00 uM |
* Present only in M222- 1ML
M250-1ML, Mammalian Protease Inhibitor Cocktail:
Preparation of 100X Solution:
1. Add 1 ml deionized, distilled water and mix gently until powder is
completely resuspended.
2. Aliquot into multiple tubes and store at -20ºC.
|
Name |
MW |
100X Concentration |
Recommended
Working Concentration |
|
AEBSF |
239.5 |
120.00 mM |
1.20 mM |
|
Aprotinin |
6512.0 |
46.00 uM |
0.46 uM |
|
Bestatin |
308.4 |
1.36 mM |
14.00 uM |
|
E-64 |
357.4 |
1.23 mM |
12.30 uM |
|
Leupeptin |
493.6 |
11.20 mM |
112.00 uM |
|
Pepstatin |
685.9 |
116.00 uM |
1.16 uM |
M306-5ML Bacterial Protease Inhibitor Cocktail
Preparation of 20X Solution:
1. Add 1 ml DMSO to vial and vortex 1 minute.
2. Add 4 ml deionized, distilled water and mix.
3. Aliquot into multiple tubes and store at -20ºC.
|
Name |
MW |
20X Concentration |
Recommended
Working Concentration* |
|
AEBSF |
239.5 |
22.00 mM |
1.10 mM |
|
Bestatin |
308.4 |
1.87 mM |
9.35 uM |
|
E-64 |
357.4 |
220.00 uM |
11.00 uM |
|
Pepstatin |
685.9 |
2.20 mM |
110.00 uM |
|
EDTA |
372.2 |
93.00 mM |
4.65 mM |
M307-1ML, Plant Protease Inhibitor Cocktail:
Preparation of 100X Solution:
1. Add 1 ml dimethyl sulfoxide (DMSO, Code: 0231) and mix gently until
powder is completely resuspended.
2. Aliquot into multiple tubes and store at -20ºC.
|
Name |
MW |
100X Concentration |
Recommended
Working Concentration |
|
AEBSF |
239.5 |
220.00 mM |
2.20 mM |
|
Bestatin |
308.4 |
10.70 mM |
107.00 uM |
|
E-64 |
357.4 |
3.00 mM |
30.00 uM |
|
Leupeptin |
493.6 |
2.00 mM |
20.00 uM |
|
Pepstatin |
685.9 |
2.20 mM |
22.00 uM |
|
1,10-Phenanthroline |
198.2 |
550.00 mM |
5.50 mM |
|
